Characterization of the O-Glycoproteome of Flavobacterium johnsoniae

Paul DD Veith; Dhana GG Gorasia; Eric CC Reynolds

Journal article

Characterization of the O-Glycoproteome of Flavobacterium johnsoniae

Paul DD Veith, Dhana GG Gorasia, Eric CC Reynolds

Journal of Bacteriology | American Society for Microbiology | Published : 2023

DOI: 10.1128/jb.00093-23

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Abstract

Flavobacterium johnsoniae is a free-living member of the Bacteroidota phylum that is found in soil and water. It is frequently used as a model species for studying a type of gliding motility dependent on the type IX secretion system (T9SS). O-Glycosylation has been reported in several Bacteroidota species, and the O-glycosylation of S-layer proteins in Tannerella forsythia was shown to be important for certain virulence features. In this study, we characterized the O-glycoproteome of F. johnsoniae and identified 325 O-glycosylation sites within 226 glycoproteins. The structure of the major glycan was found to be a hexasaccharide with the sequence Hex–(Me-dHex)–Me-HexA–Pent–HexA–Me-HexNAcA. B..

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University of Melbourne Researchers

Paul Veith Author

Dhana Gorasia Author

Eric Reynolds Author

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Grants

Awarded by Australian National Health and Medical Research Council

Awarded by Australian Research Council

Awarded by Australian Government Department of Industry, Innovation, and Science

Funding Acknowledgements

We thank Susan Veith for research assistance and the staff of the Mass Spectrometry and Proteomics Facility at the Bio21 Institute at the University of Melbourne (Parkville, Australia) for the acquisition of Orbitrap LC-MS/MS data and related technical support.This work was supported by the Australian National Health and Medical Research Council (grant 1123866), the Australian Research Council (grant DP200100914), and the Australian Government Department of Industry, Innovation, and Science (grant 20080108).